摘要
目的:了解十堰地区铜绿假单胞菌β-内酰胺类药物的耐药表型和基因分布情况,并探讨基因型与表型的相关性。方法:用VITEK 2 Compact高级专家系统判定2011年1～9月分离的896株铜绿假单胞菌对β-内酰胺类药物的耐药表型,用PCR检测25株铜绿假单胞菌的13种β-内酰胺酶基因。结果:896株铜绿假单胞菌对β-内酰胺酶类药物的耐药表型有9种,连续分离的25株铜绿假单胞菌中耐药表型有4种,主要表型均为高水平耐药+碳青霉烯酶(不渗透性),分别占47%和48%。检出阳性的8种耐药基因包括bla OXA-10、bla DHA、bla SHV、blaCARB、bla GIM、bla GES、bla TEM,阳性率分别为4%、4%、20%、12%、8%、8%、16%,另bla oprD2基因缺失率为60%,未检出bla IMP、bla VIM、bla PER、bla VEB和bla SPM。结论:本院铜绿假单胞菌对β-内酰胺类抗菌药物耐药与携带bla OXA-10、bla DHA、bla SHV、bla CARB、bla GIM、bla GES、bla TEM基因以及膜孔蛋白基因oprD2基因缺失有关,这也是本地区β-内酰胺类药物耐药的主要原因。
Objective To investigate the β-lactam antibiotic resistant phenotype and genetypes of Pseudomonas aeruginosa(Pae) in Shiyan district.Methods The senior expert system of VITEK 2 Compact was used to judge the β-lactamase drug resistant phenotypes in 896 strains Pae from January to September in 2011.Total 13 kinds of β-lactamase genes were detected by PCR in the consecutively separated 25 strains Pae from July to September.Results There were 8 species of β-lactam antibiotic resistant phenotypes in 896 strains and 4 species in 25 strains,in which the main phenotype was high level R+R Carbapenems(IMPER) and accounted for 47% and 48%,respectively.The positive β-lactamase genes were bla OXA-10,blaDHA,bla SHV,bla CARB,bla GIM,bla GES,bla TEM,and the positive rates were 4%,4%,20%,12%,8%,8%,16%,respectively,and the lacking rate of bla oprD2 gene was 60%.No bla IMP,bla VIM,bla PER,bla VEB and bla SPM genes were detected.Conclusion The β-lactam antibiotic resistant of Pae were correlated with the genes of bla OXA-10,bla DHA,bla SHV,bla CARB,bla GIM,bla GES,bla TEM and oprD2 gene lacking,which were the main reasons for β-lactam antibiotic resistance in Shiyan district.
出处
《湖北医药学院学报》
CAS
2011年第6期577-580,共4页
Journal of Hubei University of Medicine
基金
十堰市科技局科技攻关计划项目(2010st05)
