摘要
本文报导了用我国狂犬病毒固定毒人二倍体细胞适应株（ＣＴＮ－１）进行Ｖｅｒｏ细胞适应传代研究。通过连续传代培养，滴度可达８．０ｌｏｇＬＤ_（５０）ｍｌ，达到了ＷＨＯ规定的不需浓缩的标准。病毒用０．０１ＭＯＩ感染细胞其产量与１Ｍｏｌ感染量相仿。病毒增殖高峰在４－５天，维持达１５天无明显下降，且可连续收获４－５次。因此，该毒种符合ＷＨＯ提出的疫苗生产毒种要求，可用于狂犬病疫苗生产。
The purified Vero cell rabies vaccine is one of the most successfuly developed new rabies vaccine. It's potency, stability and human trials have been shown satisfactory. We have now adapted a rabies virus strain in Vero cells. The results showed as follows. The CTN-1 (37) strain of rabies virus were adapted in Vero cell by consecutive passage. The titer of virus on first passage was 7. 0 Log LD_(50)/ml after adaptation it reached to 8.0 Log LD50/ml in the 20th passage, The virus yeild with 0. 01 M. O. I. inoculation in Vero cell was similar with 1. 0 M. O. I. The peak of virus titer was found in day 4-5. Several harvests of virus fluid with similar high titer could be obtained beginning from day 5 to day 15. It is considered that this adapted strain could be used as vius seed for Vero cell rabies vaccine.
出处
《微生物学免疫学进展》
1995年第2期82-85,共4页
Progress In Microbiology and Immunology
