摘要
以大岩桐（ＳｉｎｎｉｇｉａｓｐｅｃｉｏｓａＢｅｎｔｈ．ｅｔＨｏｏｋ）叶片为试材，接种于ＭＳ附加不同激素组合的培养基上，成功地经愈伤组织亚球状体至不定芽途径形成大量再生植株并移栽成活。在各种细胞分裂素中，６－苄基嘌呤（ＢＡ）的效果优于激动素（ＫＴ）。生长素萘乙酸（ＮＡＡ）与ＢＡ及ＫＴ配合使用时愈伤组织诱导、球状体分化及不定芽增殖均有明显的增效作用。其最适宜的培养基组成为：ＭＳ＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ在这个培养基上，愈伤组织诱导率为９４％，球状体分化率为１８８％，不定芽在口周内增殖５倍。最适宜的生极培养基为：ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ，生根率在９０％以上，试管苗移栽成活率在８０％以上。
Leaves of Sinningia speciosa senth. et Hook were inoculated on MS solid media supplied with different combinations of phytohormone, and a large amount of plantlets were regenerated via. callus to globoids to adventitious bud and were successfully transplanted to soil.The effects of BA was superior to that of KT among cytokinins. Cullus induction, globoidsdifferentiation and adventitious bud multiplication were obviously promoted by the combina. non of BA or KT with NAA; The best medium was: MS+BA 2. oppm+NAA 0. Zppm, Onthis medium, the cullus induction rate was 94%, the globoid differentiation rate was 188%,the adventitious bud multiplication rate was 5 times at four week interval. The best rootingmedium was: MS+NAA 0. bppm. The rooting rate was above 90%. The rate of alive transplanted plantlets was above 80%.
出处
《生物技术》
CAS
CSCD
1995年第2期22-24,共3页
Biotechnology
关键词
大岩桐
愈伤组织
球状体
叶片培养
花卉
Sinningia speciosa Benth. et Hook
Callus
Globoid
Adventitious bud
