摘要
研究了辣根过氧化物酶在三种表面活性剂（ＳＤＳ，ＴｒｉｔｏｎＸ－１００及ＣＴＡＢ）的水相胶束中催化联苯胺聚合反应的动力学。结果表明水相胶束体系有利于反应的进行。辣根过氧化物酶在水相胶束体系中遵循米氏反应，Ｋ_ｍ在ＳＤＳ、ＴｒｉｔｏｎＸ－１００及ＣＴＡＢ三种体系中分别为３．０１４×１０^（－４）ｍｏｌ／Ｌ、１．７２８×１０^（－４）ｍｏｌ／Ｌ和５．６６４×１０^（－５）ｍｏｌ／Ｌ。由于微环境的不同，ＨＲＰ在三种体系中表现出不同的最适反应温度和最适ｐＨ。
The kinetics of HRP oxidizing benzidine were investigated in three typical aqueous micellar systems which were formed by SDS, Triton X-100 and CTAB.The results indicated that the catalysis of HRP in these system followed Mechaelis-Menten mechanism as it did in the media of water and organic solvent. The measured K_m Values in SDS, Triton X-100 and CTAB micellar systems were 3.14 ×10￣(-4) mol/L, 1.728 ×10￣(-4)mol/L and 5.664 ×10￣(-5) mol/L, respectively.Moreover, HRP presents distinct optimal temperature and PH in these micellar systems , which may result from the disparity of microenvironment in each one.
出处
《生物化学杂志》
CAS
CSCD
1995年第6期702-705,共4页
基金
酶工程开放基金
