摘要
建立一种测定硫酸软骨素(ChS)的新方法.本方法利用4-氨基-3-肼基-5-巯基-1,2,4,-三唑(AH-MT)与硫酸软骨素反应生成紫色的衍生物,即6-巯基-3-均三唑并[4,3-b]对称四嗪,在540nm波长处测定硫酸软骨素,浓度在0.1～1.0g·L-1范围内符合朗伯比耳定律,该方法简单、快速,灵敏度高.
To establish a new method for the determination of chondroitin sulfate. The method was based on the interaction of sodium chondroitin sulfate with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to yield a violet product, 6-mercapto-3-substituted-s-triazolo-[4,3-b-]-s-tetrzaine derivatives. Absorbance at 540 nm obeyed Beer＇s Law within the concentration range from 0.1 to 1.0 g · L^-1 and the results were precise and accurate. The method was operationally simple and had a good precision and sensitivity.
出处
《中国海洋药物》
CAS
CSCD
2005年第4期21-23,共3页
Chinese Journal of Marine Drugs
基金
山东省科技厅资助项目(2001BB1CBA2)
