摘要
目的通过体外细胞培养,观察茶多酚对人甲状腺乳头状癌细胞(CGTHW3)的增殖、侵袭能力及凋亡的影响。方法用MTT法检测细胞增殖活性,观察不同浓度茶多酚对CGTHW3细胞增殖活性的影响;并通过培养板划痕法观察茶多酚对CGTHW3细胞侵袭能力的影响;应用流式细胞仪检测各浓度茶多酚诱导CGTHW3细胞凋亡的作用。结果茶多酚(50～400mg/L)抑制CGTHW3细胞增殖,其作用呈浓度、时间依赖关系。茶多酚对CGTHW3细胞的半量抑制浓度(IC50)为第1天240mg/L,第3天229mg/L,第5天200mg/L,第7天118mg/L。在细胞侵袭实验中,茶多酚可使反映细胞侵袭能力的无细胞带增宽。经茶多酚作用48h后,CGTHW3细胞凋亡百分比明显增加,对照组凋亡百分比为3.51%,100mg/L茶多酚组为12.61%,200mg/L茶多酚组为52.97%,400mg/L茶多酚组为70.79%(P<0.01)。结论茶多酚抑制CGTHW3细胞增殖。茶多酚对CGTHW3细胞的侵袭能力有一定的抑制作用。茶多酚可诱导CGTHW3细胞凋亡。
Objective To observe the effect of tea polyphenols on proliferation, invasion and apoptosis of human thyroid papillary carcinoma cells （CGTH W-3） in vitro. Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTF assay, plate scarification assay and flow cytometer, respectively. Results Tea polyphenols （50-400mg/L） inhibited the proliferation of CGTH W-3 cells with dose-and time-dependent manners. The IC50 of tea polyphenols to CGTH W-3 cells was 240 mg/L （ 1st day） , 229 mg/L （3 rd day）, 200 mg/L （5th day） and 118 mg/L （7th day）. The cell free zoster which reflected the invasive ability was widened by tea polyphenols. The apoptosis percentage of CGTH W-3 cells incubated with tea polyphenols for 48h was obviously increased, with 3.51% in control group, 12.61% with 100 mg/L tea polypheuols, 52.97% with 200 mg/L tea polyphenols and 70.79% with 400 mg/L tea polyphenols （all P〈0.01）. Conclusion Tea polyphenols inhibit the proliferation of CGTH W-3 cell in vitro. Tea polyphenols have certain inhibitory effect on invasion of CGTH W-3 cell on fibronectin-coated plate and tea polyphenols can induce apoptosis of CGTH W-3 cells.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2005年第4期366-368,共3页
Chinese Journal of Endocrinology and Metabolism
