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网状内皮组织增生症病毒中国分离株HA9901全基因组核苷酸序列的测定与分析 被引量:9

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摘要 以网状内皮组织增生症病毒(REV)中国分离株HA9901感染的鸡胚成纤维细胞(CEF)基因组DNA作为其前病毒基因组模板,根据已发表序列设计合成6对引物,经PCR扩增出6段连续的、相互部分重叠的DNA片段和闭合环形前病毒两末端LTR的连接区段,并分别连入T载体进行克隆、测序.用DNAstar软件对测序结果进行剪辑和拼接,完成了REV第一个中国分离株HA9901前病毒全基因组核苷酸序列.在从截然不同的地区、不同年份、不同禽类分离到的毒株中,将该序列与另两个毒株已完成的全基因组序列的比较表明,REV的整个基因组相对保守,各毒株间对应基因的同源性都在92%以上.其中,从我国鸡体分离到的野毒株HA9901与美国鸡源分离株FA在整个基因组上的同源性均显著高于美国的鸭源SNV株.
出处 《中国科学(C辑)》 CSCD 北大核心 2005年第4期340-348,共9页 Science in China(Series C)
基金 国家自然科学基金资助项目(批准号:30270060)
作者简介 E—mail:zzcui@sdau.edu.cn
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参考文献24

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