摘要
The application of most probable number(MPN)method in counting the numbers of photosynthetic bacteria(PSB)showed that this method was suitable for PSB counting under illumination and anaerobic condition,which remedied the shortages of other methods.With this method,the sensitivity and accuracy of the counting could be enhanced when more replicated tubes and lower dilution times for fewer bacteria samples were applied.When PSB were not dominant in soil and water,MPN method could be used instead of other methods.PSB agent could only survive 69 days,and not be dominant in the environment in a long term.Its application time and oxygen level affect the numbers of PSB significantly,which were the largest in a short application time and under anaerobic condition.
The application oi most probable number(MPN)method in counting the numbers ot photosynthetic bacteria (PSB)showed that this method was suitable for PSB counting under illumination and anaerobic condition, which remedied the shortages of other methods. With this method, the sensitivity and accuracy of the counting could be enhanced when more replicated tubes and lower dilution times for fewer bacteria samples were applied. When PSB were not dominant in soil and water, MPN method could be used instead of other methods. PSB agent couldonly survive 6-9 days, and not be dominant in the environment in a long term. Its application time and oxygen level affect the numbers of PSB significantly, which were the largest in a short application time and under anaerobic condition.
出处
《应用生态学报》
CAS
CSCD
北大核心
2005年第8期1577-1580,共4页
Chinese Journal of Applied Ecology
基金
国家"十五"重大科技攻关项目(2001BA804A43)
黑龙江省教育厅项目(10511190)
黑龙江省教育厅骨干教师创新能力资助项目(10539048)
关键词
最大或然数法
光合细菌
计数方法
沼泽红假单胞菌
Photosynthetic bacteria(PSB), Most probable number(MPN), Bacteria counting, Application effect
作者简介
崔战利,女,1964年生,硕士,副教授.主要从事土壤和环境微生物研究。发表论文12篇。E-mail:zhanlic@yahoo.com.cn。通讯联系人。