摘要
采用高效液相色谱法为定性、定量手段,研究了19℃下单次肌注给药后磺胺甲基异(口恶)唑在健康中国明对虾体内的代谢规律。给药后血淋巴内的药物浓度即达到峰值,此后迅速下降;肝胰腺内药物浓度较低,给药2小时后达到最高浓度(4.97μg/ml),此后迅速下降,至48小时不能检出。采用MCP-KP自动化药动学分析程序对数据进行分析,结果表明,中国明对虾单次肌肉注射磺胺甲基异曝唑后(50mg/kg),血药经时过程符合一级吸收二室开放模型。主要动力学参数如下:吸收半衰期(t1/2α)为0.41小时,消除半衰期(t1/2β)为10.87小时,药时曲线下面积(AUC)为64.51mg/L.小时,表观分布容积(vd)为12.21L/kg,总体清除率(CL)为780.05ml/kg/小时。结果表明,磺胺甲基异(口哑)唑在中国明对虾体内分布较广、代谢较快。
The pharmacokinetics of Sulfame thoxazole was studied in Fenneropenaeus chinensis by using the High Performance Liquid Chromatography (HPLC).The study was performed at 19 ± 1 ℃.All the shrimp received a single intramuscular administration of SMZ at a level of 50 mg/kg.After cessation of the treatment,eight shrimp were sacrificed and tissues sampled at 0.083h,0.25h,0.5h,1h,2h,6h,12h,36h and 48h.All the samples(i.e.,hemolymph,and hepatopancreas)were frozen at -20℃ until analysis. SMZ residues in samples were determined by high performance liquid chromatography(HPLC).Data were analyzed with the pharmacokinetics computer program MCP-KP. The results showed that the hemolymph concentration-time course of SMZ can be described by a two-compartment open model with the first order absorption after a single intramuscular administration. The main pharmacokinetics parameters were those:The distribution and elimination half-lives(t1/2 α and t1/2 β) were found to be 0.41 h and 1087h;The area under the drug concentration curve(AUC)was 64.51 mg/L.h;The volume distribution(Vd)was 12.21 ml/kg;The total body clearance of the drug(CL)was 780.05 ml/kg/h. The results indicated that SMZ was well distributed throughout the body,and the shorter elimination half-lives(t1/2 β)showed that the drug eliminated more quickly in Fenneropenaeus chinensis.
出处
《齐鲁渔业》
2005年第8期1-4,共4页
Shandong Fisheries
基金
国家科技攻关计划课题(2002BA906A39)国家农业科技跨越计划(2003-5)山东省科技发展计划项目(2003-4-1)等联合资助
