摘要
利用反相高效液相色谱法,简单、快速、准确地同时定量测定饲料添加剂中脂溶性维生素——V_K,V_A,V_D和V_E的含量.相邻各维生素色谱峰之间的分离度R_S在1.0～2.5之间,各个维生素的回收率在93～89％范围内.变异系数在5.9～1.2％之间.色谱条件为:μ-Bondapak C_(18)径向加压柱,流动相为甲醇,流速为0.8ml/min.紫外可变波长检测器采用MAXPL操作方式。使V_A在325nm检测;V_E在292nm检测;V_D和V_K在265nm检测.最低检出量V_A和V_D为5ng,V_K为2ng,V_E为40ng.
A revcrsed-phase high performance liquid ehromatograpby method was described for the simultaneous quantitative determination of fat-soluble vitamin K, A, D and E in feed additives. Their separation was accomplished with a 8mm×10cm μ -Bondapak C18 radial compression column and methanol eluent at flow rate 0.8 ml/min. Detection limits used UV variable wavelength detector at MAXPL mode were 5 ng for vitamin A at 325nm, 2 ng for vitamin K at 265 nm, 40 ng for vitamin E at 292 nm and 5 ng for vitamin D at 265 nm. The resolution Rs of adjacent vitamin peaks were in the range of 1.0-2.5. Recoveries of four vitamins reached 939%-89%. Coefficients of variation were between
关键词
饲料添加剂
脂容性
维生素
测定
reversed-phase high performance liquid chromatography
fat-soluble vitamin
feed additive
