摘要
花生ＤＮＡ导入大豆后，子代种子的蛋白质含量明显高出双亲，表现出杂种优势。对萌发各期子叶蛋白的电泳分析表明，导入后代的绝大部分蛋白亚基组分与大豆相似，但分子量近３０ＫＤ的一条蛋白带与花生的相似，β亚基的变化也与大豆的有差异。导入后代的甘氨酸、亮氨酸、组氨酸和苏氨酸含量明显高于两亲本，而谷氨酸、天冬氨酸、丙氨酸、缬氨酸、蛋氨酸、色氨酸低于亲本；其它氨基酸含量接近两亲本之一或居中，其中丝氨酸、异亮氨酸含量与花生的相似。结果表明：花生ＤＮＡ导入大豆后，引起了后代在蛋白质和氨基酸方面的明显变异。
Abstract Exogenous peanut (Arachis hypogaea) DNA was introduced into soybean (Glycinemax)after self-pollinated by liquid method. One of the variation types (D3) was selected and its protein and amino acids were compared with its parents. It was showed by the SDS-PAGE that most of the protein subunits in D3 cotyledon were similar to that in soybean during different germination stages, while a subunit with molecular weight abour 30000 Doldon was obviously came from peanut,yet the changes of β-subunits in D3 during germination were different from that in soybean. The contents of Gly,Leu,His and Thr in D3 were extremely higher than that in its parents, while the contents of Glu, Asp, Ala, Val,Met,and Trp were lower than their parents. The contents of Ser and lie were the same as those in peanut. The results showed that introducing peanut's DNA into soybean did induce the difference in their offsprings.
出处
《大豆科学》
CSCD
北大核心
1995年第4期316-320,共5页
Soybean Science
