摘要
从河南省西峡晚白垩世一枚恐龙蛋化石内腔絮状内含物及外壳提取ＤＮＡ样品，并用一段特异引物与６碱基随机引物进行ＰＣＲ扩增、基因克隆和序列分析。结果表明用这对引物从蛋腔絮状内含物样品中扩增到一段１５０ｂｐ的ＤＮＡ片段，推测氨基酸序列与ＥＭＢＬ库中已知蛋白质氨基酸序列进行了序列同源性分析，结果表明所克隆到的这段基因与非洲爪蟾表皮钙粘着蛋白（ｃａｄ－ｈｅｒｉｎ）前体及脑细胞粘着蛋白、牛及人胎盘胞钙粘着蛋白在氨基酸水平有显著的序列同源性，分别为４０．６％，３７．５％，３４．４％和３６．４％。
DNA was extracted from 70～80 million year old dinosaur egg excavat-ed from Cretaceous belt formation in Xixia Henan.China.The DNA samples from both outer shell and inner floccule contents were used as templates in polymerase chain reactions (PCR )with a specific primer and hexamers. With these two primers a l50 bp DNA fragment has been amplified , cloned and sequenced.Deduced amino acid sequence of this dinosaur DNA has been compared with known protein se-quences in EMBL Bank. The results showed that it has significant homology with Xenopus laevis epithelia precursor-Ep-cadherin(40.6%)and brain-cadherin(37.5%)、bovine placental cadherin(34.4%)and human placental cadherin(36;4%),respectively.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1995年第2期148-152,共5页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
北京大学和蛋白质工程及植物基因工程国家重点实验室资助
