摘要
作者采用原位杂交法检测了大鼠严重烫伤后肿瘤坏死因子ｍＲＮＡ（ＴＮＦｍＲＮＡ）在肝脏中的表达及细胞定位。结果发现，在正常大鼠肝脏中的枯否氏细胞就表达ＴＮＦｍＲＮＡ。烧伤后，ＴＮＦｍＲＮＡ表达阳性细胞数迅速增加。伤后６～１２小时达顶峰，２４小时与正常无明显差异。其动态变化与门静脉血浆内毒素的变化极为相似。同时还发现烧伤后血窦内皮细胞及门静脉周围浸润的炎症细胞ＴＮＦｍＲＮＡ表达也为阳性，其变化以伤后１２～２４小时最为明显。作者认为肠源性内毒素是ＴＮＦｍＲＮＡ表达的主要刺激物。内皮细胞、中性粒细胞表达阳性提示其已被活化。上述三种细胞在内毒素导致的肝脏损害中发挥了重要作用。
Abstract hsitu hvbrMization was used to study the expression and localization of tumor necrosis factor(TNF)mRNA in liver of rats aftcf bufn.The results showcdthat the KupHer cells expressed TNF mRNA in normalrats。 After burn,the number of positive expressioncells increased quickly,the expression reached max-mum at 6h postburn,and there were no significantlydifferent with normal at 24h postburn。The dynamicchanges of number of TNF mRNA producing cells wassimilar to that of the portal plasma endotoxin level。Kupffer cells located in sinusoid surrounding portalvein expressed TNF mRNA especially at 6h～12h postburn。Also the sinusoid endothlial cells and infiltrativeinflammation cells surounding portal vein were theTNF mRNA-producing cells,this changes appearedsignificantly at 12～24h postburn; The resuIts sug-gested that the endotoxin originated from gut may bethe prinicipal stimulator for expression of TNF mRNA。 The expression of endothlial cells and neutrophilssuggested that these cells had been activated. So theendothlium,neutrophils and Kupffer cell play an im-portant role in the pathogenesis of the endotoxin-in-duced live inlurv。
出处
《中华外科杂志》
CAS
CSCD
北大核心
1995年第10期636-638,共3页
Chinese Journal of Surgery
基金
国家自然科学基金
