摘要
目的:了解HBV慢性感染免疫耐受期患者肝内T、B 淋巴细胞的变化特点,探讨肝内免疫活性细胞与HBV 慢性感染免疫耐受的关系. 方法:采用免疫组织化学方法检测16例HBV感染免疫耐受期患者肝组织内T、B淋巴细胞在肝内分布情况, 及HBsAg、HBcAg在肝细胞内表达状况,并与6例正常肝组织、17例免疫活动期患者进行比较. 结果:免疫耐受期患者肝内浸润的CD3+、CD4+、CD8+ T淋巴细胞数明显多于正常肝组织(t=4.504,8.272, 4.742,P均<0.01),但明显少于免疫活动期患者(t= 7.079,6.866,6.316,P均<0.01);疫耐受期患者肝脏汇管区CD4/CD8比值小于免疫活动期患者(t=3.46,P<0.05), 与正常肝组织比较无差异(P>0.05);免疫耐受期患者肝内B细胞数明显少于免疫活动期患者(t=4.76,P<0.01), 但与正常组比较均无差异(P>0.05);免疫耐受期患者肝细胞内HBcAg阳性表达明显高于免疫活动期患者(t= 8.76,P<0.01). 结论:肝组织内CD3+、CD4+、CD8+T淋巴细胞及B 淋巴细胞在慢性乙型肝炎的肝组织炎症损伤及肝内HBV 清除中起重要作用;免疫耐受期患者肝组织内T淋巴细胞、B淋巴细胞浸润量低,与免疫耐受有一定的关系.
AIM: To investigate the characteristics of intrahepatic CD3+, CD4+, CD8+ T lymphocytes and B cells in immune tolerant phase of patients with chronic hepatitis B virus infection, and to explore the relationship between intrahepatic immune competent cells and the status of immune tolerance in chronic HBV infection. METHODS: Sixteen liver biopsies were selected from patients with HBV infection in immune tolerant phase for the immunohistochemical detection of T lymphocyte subsets, B lymphocytes and expression of HBsAg and HbcAg. Six normal liver biopsies and 17 liver biopsies of patients in immuneactive phase were employed to serve as control respectively. RESULTS: CD3+, CD4+, CD8+ T lymphocyte subsets in the liver biopsies of patients in immune tolerant phase were all significantly increased as compared with those in the normal control (t= 4.504, 8.272, 4.742, all P<0.01), but significantly decreased as compared with those in biopsies of patients in immuneactive phase (t=7.079, 6.866, 6.316, all P<0.01). The ratio of CD4/CD8 in portal tract was lower in immune tolerant phase than in immuneactive phase (t= 3.46, P<0.01), but it was not markedly different from that in the normal control. The number of intrahepatic B cells in immune phase was decreased statistically as compared with that in immuneactive phase (t= 4.76,P<0.01), but were almost the same as that in normal control (P>0.05). The rate of HBcAg expression in hepatocytes of patients at immune tolerant phase was significantly higher than that of patients at immuneactive phase (t = 8.76, P<0.01). CONCLUSION: T and B lymphocytes may play important roles in the clearance of HBV as well as in liver inflammation and injury during chronic HBV infection. The fewer T and B lymphocyte infiltrations are associated with the status of immune tolerance.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第13期1529-1534,共6页
World Chinese Journal of Digestology
