摘要
目的研究p16在胆管癌中的表达并探讨其临床意义。方法应用超敏S-P免疫组化方法检测38例胆管癌和8例胆总管囊肿中p16蛋白的表达情况。结果p16蛋白在胆管癌中的表达阳性率为57.89%,低于在胆总管囊肿中的表达阳性率100%,有显著统计学差异(P<0.05);p16蛋白阳性率在胆管癌Ⅰ～Ⅲ级逐级下降,Ⅰ级的阳性率高Ⅲ级(P<0.05);在Ⅰ～Ⅳ期阳性率逐期下降,Ⅰ+Ⅱ期的阳性率高于Ⅲ+Ⅳ期的阳性率(P<0.05);有淋巴结转移癌组织中的表达阳性率为31.25%,显著低于在无淋巴结转移癌组织中的阳性率77.27%(P<0.05)。结论p16可能参与了胆管癌的发生、发展和转移,检测p16蛋白的表达对评估胆管癌的分期、分级及淋巴结转移有重要意义。
Objective To investigate the expression and clinical significance of p16 in cholangiocarcinoma. Methods Supersensitive S-P immunohistochemical method was used to detect the expression of p16 proteins in 38 cases of cholangiocarcinoma and 8 cases of biliary cyst. Results The positive rates of p16 were 57.89% in cholangiocarcinoma and 100% in biliary cyst. p16 positive expression rates in cholangiocarcinoma were significantly lower than those in biliary cyst (P<0.05). The expression level of p16 proteins significantly correlates with tumor grade, clinical stage and lymph node metastasis (P<0.05).Conclusion p16 has close relationship with tumorgenesis, progression and malignant behavior in cholangiocarci noma.
出处
《菏泽医学专科学校学报》
2005年第3期10-11,共2页
Journal of Heze Medical College
关键词
胆管癌
P16
免疫组化
cholangiocarcinoma
p16
immunohistochemistry
