摘要
目的选取了自发性高血压大鼠(SHR)与肾性高血压大鼠(RHR)作为肥厚心肌的供体,从整体水平检测了时钟体系中最为重要的BMAL1蛋白在3种不同心肌中的表达。方法建立了肾性高血压大鼠的模型,取自发性高血压大鼠,肾性高血压大鼠以及正常大鼠作为对照组,在每12h光暗交替的环境中饲养2wk后,每隔4h取心脏,测心重指数等各项指标,并用Westernblot方法检测高血压大鼠心肌组织与正常大鼠心肌组织中BMAL1蛋白表达的变化。结果RHR和SHR大鼠的左心重与全心重比值及全心重与体重比值与正常组相比差异都具有显著性,正常大鼠心肌组织中BMAL1蛋白表达呈现出明显的节律性,但RHR与SHR组心肌组织中的BMAL1蛋白表达丧失了应有的波动性与节律性,各时间点之间几乎无差异。结论在心肌肥厚的发病过程中时钟基因可能起一定的作用,时钟基因蛋白表达的改变可能是影响肥厚心脏逐渐丧失适应能力的一个因素。
Aim To explore the expression of BMAL1 protein in the myocardiums from three kinds of rats(SHR,RHR,NORMAL) respectively. Method The model of renal hypertensive rat (RHR) was established,SHR,RHR and NORMAL rats were housed in a separate environment-controlled room for two weeks,in which a strict 12-hour light/12-hour dark cycle regimen was enforced(lights on at 21:00,zeitgeber time 0).On the day of experiment,rats were killed every four hours from 17:00 pm to 5:00 am.Results BMAL1 protein expression exhibited a rhythm of approximately 24 hours in normal myocardium, but its expression in hypertrophic myocardia loses this rhythm. Conclusion These data suggest that clock gene may play a role in the development of hypertrophy. Abnormal expression of clock protein may cause the heart unable to adapt to the ever-changing environment.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第7期811-815,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30271506)
