摘要
试验结果表明脂肪氧合酶最适反应温度为30℃,最适pH为10.0和4.0。在pH10.0的条件下,最适底物浓度为2.5×10-4mol/L,Km值为0.073mmol/L,Vmax值为3.08×104U/mgprotein.min。EDTA对该酶有较强的抑制作用。而在pH4.0的条件下,最适底物浓度为5×10-4mol/L,BHA、BHT、TBHQ等抗氧化剂有较强的抑制作用。
Characterization of a lipoxygenase were carried out from Nile tilapia Oreohromis niloticus gills. The results showed that the enzyme had optimal temperature of 30℃,and optimal pH of 10.0 and 4.0. There were at least two isoforms in this enzyme. The optimal substrate concentration was 2.5×10 -4 mol/L at pH 10.0, and Km and Vmax were 0.073 mmd/L and 3.08×104 U/mg protein.min, respectively. EDTA was a better inhibitor. The optimal substrate concentration was 5×10 -4 mol/L for the lipoxygenase activity at pH 4.0. Anti-oxidants such as butylhydroxyanisol (BHA), butylated hydroxyl toluene (BHT) and TBHQ had strong inhibit effects.
出处
《水产科学》
CAS
北大核心
2005年第7期15-19,共5页
Fisheries Science
关键词
脂肪氧合酶
罗非鱼
鳃
lipoxygenase
Nile tilapia Oreohromis niloticus
gill
characterization
