摘要
目的探讨环腺苷酸(cAMP)类似物8-溴-环腺苷酸(8-Br-cAMP)对人原代培养的膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达的影响。方法原代培养膀胱癌细胞,采用完整细胞免疫组化、原位杂交方法检测膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA的表达。结果8-Br-cAMP处理后的膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达水平明显低于未处理组,两组相比差异均有统计学意义(P<0.05)。结论8-Br-cAMP可抑制膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达,进而抑制肿瘤细胞的分裂和增殖。
Objective To study the effects of 8-Br-cAMP on the expression of c-erbB-2,c-myc gene and mRNA in bladder carcinoma cells. Methods After human bladder carcinoma cells were cultured primarily in the media containing 10-5mol/L of 8-Br-cAMP ,the alteration of expression of c-erbB-2,c-myc gene and mRNA were observed by using immunohistochemistry SP method and in situ hybridization. Results The expression of c-erbB-2,c-myc mRNA and protein in experimental group were weaker than that in control group , there was significant difference between control group and experimental group (P<0.05). Conclusion 8-Br-cAMP could inhibited the expression of c-erbB-2,c-myc and mRNA in bladder carcinoma cells, it indicated that 8-Br-cAMP was an effective inhibitor for mitotic and proliferation of tumor cells.
出处
《医药论坛杂志》
2005年第13期7-8,10,共3页
Journal of Medical Forum
基金
河南省科委科技攻关计划项目(0324410004)
