摘要
背景:臭牡丹属马鞭草科植物,现代药理学研究表明臭牡丹具有抗炎,抗肿瘤,提高机体非特异性免疫,兴奋子宫圆韧带肌电作用与激动α肾上腺受体有关,关于镇痛作用的实验研究较少。目的:通过热板法和扭体法观察臭牡丹的乙醇提取物对小鼠的镇痛作用。设计:以动物为观察对象,随机对照实验。单位:赣南医学院的药理学教研室。材料:实验于2004-03/06在赣南医学院药理教研室完成,选择昆明种小白鼠120只,体质量(20±2)g,由赣南医学院实验中心提供。干预:50只小白鼠用于扭体法实验,随机分为生理盐水组,氨基比林0.1g/kg组,吗啡0.01g/kg组,臭牡丹提取物20g/kg组,臭牡丹提取物40g/kg组,每组10只,分别经腹腔注射给药,40min后腹腔注射6mL/L乙酸溶液(10mL/kg),5min后开始观察小鼠扭体数和扭体抑制率,共观察10min。40只小白鼠用于热板法实验,随机分为生理盐水组,吗啡0.01g/kg组,臭牡丹提取物20g/kg组,臭牡丹提取物40g/kg组,每组10只,经腹腔注射给药后即刻置于热板金属表面,温度控制在(55±0.5)℃,分别记录给药后15,30,60min痛阈值。30只小白鼠用于对抗纳洛酮的热板法实验,随机分为纳洛酮0.04g/kg+吗啡0.01g/kg组,纳洛酮0.04g/kg+臭牡丹提取物40g/kg组,纳洛酮0.04g/kg+生理盐水组,经腹腔注射给药,记录给药后15,30,60,90min痛反应时间值。主要观察指标:①小白鼠扭体次数和扭体抑制率。②痛反应时间。③纳洛酮对抗后,痛反应时间。结果:120只小白鼠全部进入结果分析。①扭体次数和扭体抑制率:通过腹腔注射臭牡丹提取物20和40g/kg后小白鼠的扭体次数为2.4±2.5,0.6±1.7,它们对6mL/L乙酸溶液引起的疼痛反应的抑制率为93.3%,98.3%。②热板法实验给药后15,30,60min的痛阈值:给予臭牡丹提取物20g/kg后15,30,60min痛阈值为[(121.2±98.7)s,(191.2±78.6)s,(133.1±91.1)s];给予臭牡丹提取物40g/kg后15,30,60min痛阈值为[(233.9±70.4)s,(219.6±78.2)s,(218.3±92.6)s],均高于生理盐水组[(13.7±15.2)s,(9.7±12.5)s,(22.1±15.6)s](P<0.01～0.001)。③在纳洛酮对抗吗啡的实验中,吗啡0.01g/kg+纳洛酮0.04g/kg组给药后15,30,60min的痛域明显小于臭牡丹提取物40g/kg+纳洛酮0.04g/kg组[(1.7±5.2)s,(124.9±79.4)s,P<0.001;(6.4±8.6)s,(139.3±72.9)s,P<0.001;(21.8±34.0)s,(137.9±60.8)s,P<0.001]。结论:臭牡丹的乙醇提取物具有很强的镇痛作用,这种镇痛作用不是通过激动阿片受体而发挥镇痛效应的。
BACKGROUND:Clerodendron bungei steud(CBS)is a tree from genera of Verbena L.In the present pharmacological studies,CBS has showed antiinflammatory,antineoplasic,nonspecific immunityenhancing effects. Myoelectric effect of stimulating uterus round ligament is related with agitating adrenergic αreceptor.The experiments about analgesic effect are few.OBJECTIVE:To observe the antinociceptive effect of ethanol extract of CBS in mice through hot plate and writhing tests.DESIGN:Randomized controlled experiment with animals as subjects.SETTING:Departmcent of Pharmmacology, Gannan Medical College.MATERIALS:The experiment was performed in Department of Pharmacology,Gannan Medical College from March to June 2004. A total of 120 Kunming white mice,weighing(20±2) g were provided by the Experiment Animal Center of Gannan Medical College.INTERVENTIONS:In the writhing test, 50 mice were randomly divided into normal saline,aminopyrine group(0.1 g/kg),morphine group(0.01 g/kg)and extract of CBS groups(20 g/kg,40 g/kg).There were 10 mice in each group.Forty minutes after intraperitoneal injection.6 mL/L acetic acid(10 mL/kg) was injected intraperitoneally,5 minutes later,the number of writhing body and inhibitory rate of writhing body were observed for 10 minutes.In hot plate test, 40 mice were randomly divided into normal saline group, morphine group(0.01 g/kg)and extract of CBS groups(20 g/kg,40 g/kg).There were 10 mice in each group. After intraperitoneal injection, the mice were put on the hot plate, and the temperature was(55±0.5) °C.The pain threshold was recorded 15,30 and 60 minutes after administration.And 30 white mice were randomly divided into naloxone 0.04 g/kg+morphine 0.01 g/kg group,aloxone 0.04 g/kg+extract of CBS 40 g/kg group,naloxone 0.04 g/kg+normal saline group for hot plate test antagonized by naloxone(40 mg/kg) .The mice were injected intraperitoneally.The duration of pain reaction were recorded 15,30,60 and 90 minutes after administration. MAIN OUTCOME MEASURES:①The number of writhing body and inhibitory rate of writhing body;②The duration of pain reaction;③The pain threshold in the hotplate test after antagonized by naloxone were calculated. RESULTS:All the 120 white mice entered the result analysis.①The number of writhing body and inhibitory rate of writhing body:The number of writhing body was 2.4±2.5 and 0.6±1.7 in the 20 and 40 g/kg extract of CBS injection group respectively and the inhibitory rate of the pain reaction caused by 6 mL/L acetic acid was 93.3%and 98.3%respectively.②The pain threshold at 15, 30 and 60 minutes after administration in the hot plate test:the pain threshold was[(121.2±98.7)s,(191.2±78.6) s,(133.1±91.1) s] at 15, 30 and 60 minutes after injection of 20 g/kg extract of clerodendron bungei steud;it was[(233.9±70.4) s,(219.6±78.2) s,(218.3±92.6) s at 15,30 and 60 minutes after 40 g/kg extract of CBS being given.The pain threshold in the extract of CBS groups was all higher than that in the control group respectively[(13.7±15.2)s,(9.7±12.5)s,(22.1±15.6) s](P< 0.01-0.001) .③In naloxone antagonized morphine test, the pain threshold at 15,30 and 60 minutes after administration in morphine 0.01 g/kg+naloxone 0.04 g/kg group[(1.7±5.2) s (6.4±8.6) s,(21.8±34.0) s]was obviously smaller than that in the extract of CBS 40 g/kg +naloxone 0.04 g/kg group[(124.9±79.4)s, (139.3±72.9) s,(137.9±60.8) s](P< 0.001).CONCLUSION:Ethanol extract of CBS has strong analgesic effect and this is not manifested through the activation of opioid receptors.
出处
《中国临床康复》
CSCD
北大核心
2005年第21期250-251,共2页
Chinese Journal of Clinical Rehabilitation
