摘要
以光叶石楠Photiniaglabra当年生枝的茎段为材料,进行植物生长调节物质对光叶石楠腋芽诱导、增殖、分化、生长及生根的对比试验。结果表明:MS+0.5mg.L-1BA+1.0mg.L-1KT+0.1mg.L-1NAA为腋芽诱导的最适培养基,腋芽诱导率达93.3%;MS+1.0mg.L-1BA+0.1mg.L-1NAA为芽增殖最适培养基。1/2MS+0.1mg.L-1IBA和1/2MS+0.1mg.L-1NAA为光叶石楠试管苗生根较为合适的培养基,生根率分别为83.3%和93.0%,试管苗移栽成活率达90%以上。
With sections of newly spouted shoots of Photinia glabra as explants, the influences of plant growth regulating substances on induction, proliferation and differentiation of axillary buds, growth and rooting were studied. The results showed that the medium of MS + 0.5 mgL^(-1) BA + 1.0 mgL^(-1) KT + 0.1 mgL^(-1) NAA was optimum for induction of axillary buds, with 93.3 % buds induced; The medium of MS + 1.0 mgL^(-1)BA + 0.1 mgL^(-1) NAA was optimum for bud proliferation, and the number of buds obviously proliferated with the increase of the frequency of successive transfer culture in the scope of this test; The medium of 1/2MS + 0.1 mg L^(-1) IBA and the medium of 1/2MS + 0.1 mgL^(-1) NAA were both optimum for rooting, with the rooting rate of 83.3% and 93.0% respectively. The survival rate of transplant of tube plantlets was high than 90%.[Ch,3 tab.5 ref.]
出处
《浙江林学院学报》
CAS
CSCD
北大核心
2005年第2期238-240,共3页
Journal of Zhejiang Forestry College
基金
国家科技部"948"项目(2003-39)
关键词
育苗
光叶石楠
组织培养
植物生长调节物质
<Keyword>nursery stock growing
Photinia glabra
tissue culture
plant growth regulating substances
