摘要
目的:研究以 HPLC 同时测定半夏药材中次黄嘌呤核苷、鸟嘌呤核苷含量的方法。方法:采用 Kromasil C_(18)柱(4.6mm×150mm,10μm),以甲醇-水(1∶99)为流动相,流速1.0mL·min^(-1),λ_1=248nm(检次黄嘌呤核苷),λ_2=254nm(检鸟嘌呤核苷),柱温30℃。结果:次黄嘌呤核苷在20-421ng 范围内线性关系良好,r=0.9999,鸟嘌呤核苷在40-339ng 范围内线性关系良好,r=0.9996。平均加样回收率(n=9):次黄嘌呤核苷为99.2%;鸟嘌呤核苷为99.7%。结论:本法操作简便,结果可靠,重现性好,可作为半夏药材质量控制的方法。
Objective:To establish an HPLC method for determination of inosine and guanosine in Rhizoma Pinel- lia ternata.Methods:A Kromasil C_(18) column(4.6 mm×150mm,10μm)was used.The mobile phase was metha- nol-water(1:99),the flow rate was 1.0 mL·min^(-1),λ_1=248 nm(detect the inosine),λ_2=254 nm(detect the guanosine),the column temperature was 30℃.Results:The linear range of inosine was within 20-421ng,r= 0.9999,recovery was 99.2%(n=9).The linear range of guanosine was within 40-339 ng,r=0.9996,recovery was 99.7%(n=9).Conclusion:This method has good reproducibility.It can be used for quality control of Rhizo- ma Pinellia ternata.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第5期487-489,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家"九五"攻关项目(96-903-02-03)
