摘要
目的 了解临床分离的对亚胺培南耐药的铜绿假单胞菌消毒剂及β内酰胺类抗生素耐药相关基因存在状况。方法 自临床分离2 8株对亚胺培南耐药铜绿假单胞菌,采用聚合酶链反应(PCR)及序列分析的方法检测耐药基因。结果 2 8株中,qac E△1、TEM、OXA基因阳性株数(% )分别为2 4株(85 .7% )、2 0株(71.4 % )、1株(3.6 % ) ,此株OXA基因经序列分析确认为OXA-10型超广谱β内酰胺酶(Gen Bank注册号:AY84 185 9)。2 5株(89.3% ) opr D基因缺失,IMP、VIM、SHV、PER、VEB、GES、MIR、DHA基因均阴性。结论 临床分离的对亚胺培南耐药的铜绿假单胞菌耐消毒剂基因qac E△1及TEM基因携带率均很高,至少存在TEM、OXA- 10两种β内酰胺酶基因,opr D基因缺失突变是对亚胺培南耐药的重要原因。
Objective To investigate the drug-resistant genes associated disinfectants and β-lactam antibiotics in Pseudomonas aeruginosa resistanted to imipenem isolated from the PLA 98th Hospital,Huzhou, Zhejiang province. Methods 28 strains of Pseudomonas aeruginosa were isolated from hospitalized patients,and drug-resistant genes were analyzed by PCR and verificated by DNA sequencing. Results The positive rates of qacE△1,TEM,OXA genes in 28 strains of P. aeruginosa were 85.7%,71.4%,and 3.6%,respectively. The OXA gene was OXA-10 subtype(GenBank accession number:AY841859).The oprD gene was lack in 25(89.3%)isolates.Genes of IMP,VIM,SHV,PER,VEB,GES,MIR,and DHA were not found in all 28 isolates tested.Conclusions There are very high positive percentages of genes of both qacE△1 and TEM in Pseudomonas aeruginosa resistanted to imipenem isolated clinically, and at least 2 kinds of β-lactamases exsiste including TEM and OXA-10. The oprD gene lack are the important mechanism of Pseudomonas aeruginosa resistanted to imipenem.
出处
《东南国防医药》
2005年第3期172-175,共4页
Military Medical Journal of Southeast China
