摘要
我们以8周龄、体重20克左右的 BALB/C 小鼠为实验对象,观察正常小鼠及烫伤小鼠脾淋巴细胞经各种不同浓度精氨酸的培养液体外培养后,对 ConA 反应性的变化,同时观察经不同含量精氨酸的饮食饲养后,烫伤小鼠脾淋巴细胞功能的变化。结果表明;小鼠脾淋巴细胞经各种不同浓度精氨酸体外培养后,对 ConA 的反应性在一定的范围内随精氨酸浓度的递增而增加;烫伤小鼠通过不同含量精氨酸的摄入,其脾淋巴细胞对 ConA 的反应性呈现不同的变化,其中以摄入占总卡量2%精氨酸组的淋转功能最佳。实验提示:小鼠脾淋巴细胞的功能在一定浓度范围内对精氨酸存在剂量依赖关系,淋巴细胞最佳功能的表达需要一个最合适的精氨酸浓度.烫伤后脾淋巴细胞需要更高的精氨酸浓度才能表达其最佳功能。
It is well accepted that nutritional support improves the immunologic func- tions in burned patients.Arginine has been demonstrated to have tissue-specific properties which induce beneficial effects upon the immune system.A series of experiments are carried out,in order to evaluate the immune effect of arginine on burned mice. 1.Lymphoeytes from both burned and unburned mice are harvested and incubated in various concentrations of arginine solution for 72 hours,and ConA-stimulating lymphoeyte transformation is determined after incubation. 2.Burned animals are divided into four groups.Standard diets for nutritional support are formulated.These formulas contain an identical carbohydrate and lipid(61% and 15% of total energy respectively)intake with varied proportions of protein for each group(24%, 23%,22%,and 20% of total energy,respectively),in addition,0,1%,2% and 4% of protein energy is supplied by arginine for different groups.Lymphocyte proliferations in responses to ConA stimulation are determined on the 7th postburn day. The data shows that,in vitro study,increasing arginine concentrations enhanced lym- phocyte responses to ConA.The arginine needed level for optimal lymphocyte responses is 1.8mmol/L in burned mice and 0.9mmol/L in unburned controls.This difference indicates that in burned mice,a higher arginine concentration is required for better lymphocyte re- sponses.On the contrary,further increase of arginine concentration do not give better re- sponse.When varied amounts of arginine are given in the diet to the burned mice,the degree of lymphocyte response to ConA is different.The amount of arginine which supplies 2% of total energy is found to be optimal.
