摘要
阿胶水液(Murphy法)与其经Gornall双缩脲和Lowry酚试剂反应后的吸收光谱特征皆与参比明胶同一。以明胶作标准,用上述各光度法测定阿胶蛋白质结果彼此近似并与定氮法一致。
jiao exhibits identical
spectral characteristics with those of reference gelatin in Murphy’s
UV spectrophotometryand color-yielding reactions performed according
to Gornall’s biuret and Lowry’s Folin phenol procedures
respectively.Results of protein assays of Ejiao measured by
above-mentioned photometric methods were in gcod appro-ximation
toeach other using gelatin as standard and comparable with that of
Kjeldahl nitrogen determination.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1994年第4期224-227,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金
关键词
阿胶
蛋白质定量
明交
光度法
Ejiao
protein assay
gelatin
photometric method
nitrogen determination