摘要
基于在碱性介质中,盐酸多巴胺对鲁米诺-铁氰化钾化学发光反应体系的强烈抑制作用,建立了反相流动注射抑制化学发光测定盐酸多巴胺的新方法。研究了影响化学发光强度的因素,并探讨了化学发光反应的可能机理。该方法快速、准确、线性范围宽,测定盐酸多巴胺的检出限为1.14×10-9g·mL-1,方法的线性范围为2.0×10-9～8.0×10-7g·mL-1,对于4.0×10-7g·mL-1盐酸多巴胺测定11次的相对标准偏差为0.99%。应用于盐酸多巴胺注射液中盐酸多巴胺的测定,结果满意。
A novel chemiluminescence system coupled with a reverse flow injection analysis for the determination of dopamine hydrochloride was presented. It is based on the strong quench effect of dopamine hydrochloride on the chemiluminescence reaction between luminol and hexacyanoferrate(III) under alkaline condition. Various factors affecting the chemiluminescence intensity of the system were investigated. The possible mechanism of the proposed method was also studied. The decrease of chemiluminescence intensity was linear with the dopamine hydrochloride content in the range of 2.0 x 10(-9)-8.0 x 10(-7) g (.) mL(-1) the detection limit of the method was 1.14 X 10(-9) g(.)mL(-1), and the relative standard deviation was 0.99% (4.0 x 10(-7) g(.)mL(-1), n = 11). It was successfully used for the determination of the content of dopamine. hydrochloride in dopamine hydrochloride injection.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2005年第5期678-680,共3页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金(20277005)
山东省中青年学术骨干和学科带头人培养基金资助项目
