摘要
目的利用快速导流杂交法对人乳头瘤病毒进行快速基因分型,并探讨该技术临床应用前景. 方法 144例尖锐湿疣患者皮损脱落细胞或组织中提取DNA,PCR扩增后与9种常见HPV亚型探针进行反斑点印迹杂交(reverse dot blot, RDB)检测HPV DNA,通过与传统膜杂交结果做对比,评估导流杂交法的效果. 结果 144例样本中得到134例PCR阳性结果;HPV分型包括:高危HPV亚型16、18、31、33、58;低危亚型6、11、53;未知危险程度的亚型CP8304;68例样本中包括了2～4种亚型的混合感染. 结论两种杂交方法检测结果97%一致;快速导流法优势主要具有更高速度、操作更方便,和节省试剂用量;敏感性和特异性与传统杂交方法相同.
OBJECTIVE To type HPV using flow-through rapid hybridization technique and evaluate its reliability for clinical use. METHODS A total of 144 DNA samples were isolated from cervical condyloma tissues and used for PCR amplification. 9 types of HPV genotype were detected using flow-through hybridization technique or reverse dot blot (RDB). Compared with the conventional hybridization method, the new flow-through hybridization technique was evaluated. RESULTS After PCR amplification and hybridization, 134 samples were HPV positive. After reverse dot blot analysis, 5 high risk HPV genotypes(16, 18, 31, 33, 58), 3 low risk HPV genotypes(6, 11, 53) and CP8304 were detected in most of the samples. The multi-HPV-genotype infection was observed in 68 samples. CONCLUSIONS Identical results are observed in 97% of the samples with the two methods. Compared with the conventional method, the flow-through hybridization technique is rapid, convenient and economic. The sensitivity and specificity are the same for both methods.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2005年第6期618-621,共4页
Chinese Journal of Nosocomiology
基金
深圳市科学基金资助项目(200304017)
关键词
导流杂交
传统杂交
乳头状瘤病毒
基因分型
Flow-through hybridization
Conventional hybridization
Papillomavirus
Human genotyping
