摘要
应用１６０个ＲＦＬＰ标记分析了２１个水稻广亲和品种和６个籼粳测验种．发现其中６８个标记对以区分籼粳测验种。２１个广亲和品种根据与籼粳测验种共有片段比率的大小可以分为籼、粳和籼粳中间型三类。６８个标记在１５个籼粳品种中进一步筛选，得到２４个籼粳特异性ＲＦＬＰ标记，它们在亚种内杂文带型相同而亚种间则不一样。其中ＲＧ３５８，Ｇ３１８为籼稻专一性探针，在粳稻中发现为零等位。以此２４个探针为基础构建了广亲和品种亲缘关系的树状图。讨论了籼粳特异性ＲＦＬＰ标记在水稻遗传育种实践中的应用。
One hundred and sixty DNA clones were used to survey RFLPs among 21 wide compatibility varieties(WCVs) and 6 indica and japonica testers of Oryza sativa L. Sixty-eight clones distinguishing indica from japonica testers were detected. Based on ratios of shared fragments between WCVs and testers, 21 WCVs were divided into three groups: indica,japonica and intermediate. By further-screening among 15 indica and japonica varieties, 24 among 68 clones were found to be indica-japonica differentiating Probes. They show same hybridization patterns within subspecies, but different patterns between the subspecies. RG358, G318 were confirmed to be indica specific clones, which showed null alleies in japonica varieties. A phylogenetic tree of 21 WCVs was constructed based on these 24 indica-Japonica differentiating probes and the significance of these probes in rice genetics and breeding pyactice was discussed.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1994年第2期65-71,共7页
Chinese Journal of Rice Science
基金
浙江省自然科学基金
国家863计划资助
关键词
籼粳特异性
亲缘关系
亲和性
水稻
Indica-japonica differentiation
Phylogenetic analysis
Restriction fragment length polymorphism (RFLP)
Wide compatibility
