摘要
目的根据机体死后组织细胞DNA降解规律,研究其与死亡时间之间的关系。方法SD大鼠处死后,在不同死亡时间段取肝、肾、脾组织提取DNA。根据DNA3'末端转移的方法(TdT法)将dUTP结合到DNA的3'末端,检测反应剩余的dUTP量,从而推测机体死后不同时间段DNA的降解情况。结果经对剩余dUTP检测发现,dUTP的剩余量随死亡时间延长而减少,说明DNA降解与死亡时间存在一定的关系。结论根据机体死后DNA降解规律可用于早期死亡时间的推测。
Objective To investigate the relationship between DNA degradeation and postmortem interval. Methods dUTP was transferred to 3′terminal of DNA by using terminal deoxynucleotidyl transferase(TDT), then the reminders of dUTP after experimental reaction, as indicator of quantity of DNA degradation, were detected. Results The reminders of dUTP were decreasing along with the postmortem interval. Conclusion Postmortem DNA degradation may be used in postmortem interval judgment.
出处
《法医学杂志》
CAS
CSCD
2005年第2期113-114,共2页
Journal of Forensic Medicine
