摘要
目的比较缺氧对世居高原藏族人和移居高原汉族人脐静脉内皮细胞(UVECs)血管内皮生长因子(VEGF)、诱导型一氧化氮合酶(iNOS)和内皮型一氧化氮合酶(eNOS)mRNA表达的影响。方法分离脐静脉内皮细胞,体外原代培养,分为①世居高原藏族组和②移居高原汉族组两组,每组包括常氧对照和缺氧(0.5%O2)2h、4h、12h和24h时点。分离细胞总RNA,分别用RT-PCR检测VEGF、iNOS和eNOSmRNA表达。结果缺氧可以诱导世居高原藏族人UVECs表达iNOS和VEGFmRNA表达增加,同时抑制eNOSmRNA表达。移居高原汉族人UVECs表达上述基因mRNA的特点与世居高原藏族人相似。结论缺氧时世居高原藏族人UVECsVEGF、iNOS和eNOSmRNA表达特点与移居高原汉族人相似,提示上述基因表达的改变可能是缺氧反应机制的共同过程。
AIM: To compare the effects of hypoxia on expression of vascular endothelial growth factor (VEGF), iNOS and eNOS mRNA in cultured umbilical vein endothelial cells (UVECs) obtained from Tibetan and Han. METHODS: UVECs were obtained from native Tibetan and immigrant Han, respectively and cultured under hypoxia conditions (0.5% oxygen) for 2 h, 4 h, 12 h, and 24 h and normoxic conditions. VEGF, iNOS and eNOS mRNAs were detected with methods of RT-PCT. RESULTS: VEGF and iNOS mRNAs were up-regulated while eNOS mRNA depressed by hypoxia similarly in Tibetan and Han UVECs. CONCLUSION: Our results suggest that the changes of VEGF, iNOS and eNOS mRNA expression are common pathways in the mechanisms of hypoxic responses.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第5期844-848,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30393131)
关键词
高海拔
西藏
缺氧
内皮生长因子
一氧化氮合酶
Altitude
Tibet
Anoxia
Endothelial growth factors
Nitric oxide-synthase
