摘要
目的 探讨家兔口腔颊黏膜鳞状上皮癌前病变动物模型的建立及癌前病变的逆转方法。方法 将二甲基苯并蒽(DMBA)制成DMBA丙酮溶液及DMBA药膜,涂抹或贴附于家兔口腔颊黏膜,3次/周,共12周。癌前病变模型制成后,再贴全反式维甲酸(ATRA )药膜,3次/周,共12周,观察其转归。颊黏膜标本进行病理组织学检查,同时扫描及透射电镜超微结构观察。结果 家兔颊黏膜应用DMBA12周后均出现不典型增生,其中Ⅱ～Ⅲ级不典型增生发生率为78.5 % (11/14 )。应用全反式维甲酸12周后,颊黏膜癌前病变不同程度地逆转。结论 应用DMBA可成功构建家兔口腔颊黏膜鳞状上皮癌前病变模型,贴膜法是1种比较好局部给药方法;ATRA可能对鳞状上皮不典型增生具有一定的逆转作用。
Objective To study the establishment of a rabbit model of squamous epithelium precancerous lesion in buccal mucosa and the reverse procedure.Methods The solution or membrane containing dimethylbenzanthracene(DMBA) was pasted on rabbit's buccal mucosa three times a week for 12 weeks.Then the rabbit model with squamous epithelium precancerous lesion was developed.The membrane containing all-trans retinoic acid(ATRA) was pasted on the precancerous lesion three times a week for 12 weeks to study the reverse effect.During the whole process,histological diagnosis was performed,and ultramicroscopic structures of mucosa epithelium cells was observed by transmission electron microscope(TEM) and scanning electron microscope(SEM).Results Dysplasia was developed in all rabbits' buccal mucosa after receiving DMBA pasted for 12 weeks.The medium or severe dysplasia was 78.5%(11/14).The precancerous lesions were reversed in a variety of degree after receiving ATRA pasted for 12 weeks.Conclusion The model of squamous epithelium precancerous lesion induced by DMBA can be established in rabbit's buccal mucosa.Pasting membrane contained DMBA is a better method.ATRA may have a reverse effect on the precancerous lesion.
出处
《实用癌症杂志》
2005年第1期5-7,共3页
The Practical Journal of Cancer
