摘要
背景:阿尔茨海默病发病的自由基学说以及氧化损害与阿尔茨海默病发病的关系使抗氧化药物研究成为热点,天然中药中的抗氧自由基损伤作用是否可产生对阿尔茨海默病的干预效应?目的:观察天然中药科研制剂天泰1号对自发阿尔茨海默病模型学习记忆功能及氧自由基代谢的影响。设计:以实验动物为研究对象,完全随机分组设计的实验研究。单位:一所市级中西医结合临床研究所。材料:实验在深圳市中西医结合临床研究所二级动物实验室完成。实验动物为昆明种小鼠,清洁级,雄性,13月龄,体质量40～50g,购自中山医科大学实验动物中心(合格证号:粤检证字第95A07号)。干预:实验动物饲养至21月龄,按国际通用标准和方法从老龄动物中筛选阿尔茨海默病小鼠;实验动物随机分为空白对照组、西药对照组、天泰1号小剂量组、天泰1号大剂量组、另设老年学习记忆正常组(简称老年正常组下同)。西药对照组给以甲磺酸双氢麦角毒碱片(Hydergine)0.6mg/kg,天泰1号小、大剂量组分别予天泰1号6.80g/kg及20.41g/kg,连续60d空白对照组和老年正常组均灌以等量双蒸水。主要观察指标:用跳台实验检测学习记忆成绩;脑组织冰冻切片,免疫组织化学法示大脑皮质及海马超氧化物歧化酶活性;海马CA1区脑组织超薄切片,透射电镜法观察神经元细胞脂褐素;全自动显?
BACKGROUND:The theory of free radical on onset of Alzheimer disease(AD) and the relationship between oxidative damage and onset of AD have made anti oxidative drugs become the hot topic in researches.Whether does the action of natural Chinese herbs on anti injury of oxygenic free radical produce intervention on AD? OBJECTIVE:To observe the effects of developed and researched tiantai I,the natural Chinese herb,on learning and memory function and metabolism of oxygenic free radical in the model of spontaneous AD. DESIGN:Completely randomized experimental study based on the experimental animals. SETTING:Clinical Institute of Chinese Western integration of City grade. MATERIALS:The experiment was performed in 2nd grade Animal Experimental Room of Clinical Institute of Chinese Western integration in Shenzhen.Kunming male mice of clean grade were employed, at the age of 13 months,mass weighted varied from 40 to 50 g and from Experimental Animal Center of Zhongshan Medical University(Qualified No.95A07). INTERVENTIONS:When the experimental animals were bred till 21 months,AD mice were screened from the aged animals according to International Universal Standards and Methods.The experimental animals were randomized into blank control,western drug control,the small dosage of tiantai I group(small dosage group),the large dosage of tiantai I group(large dosage group) and learning and senile normal memory group(senile normal group) in addition.In western drug control,hydergine 0.6 mg/kg was administrated,and in small and large groups,tiantai I of 6.80 g/kg and 20.41 g/kg were administrated respectively,continuously for 60 days.The double distilled water of equal dosage was infused in both blank control and senile normal group. MAIN OUTCOME MEASURES:Step down test was applied to determine learning and memory scores.Froze sections of brain tissue were prepared and immuno histological chemical method was used to display the superoxidative dismutase(SOD) activity in cerebral cortex and hippocampus.Ultrathin section of brain tissue in hippocampal CA1 was prepared and transmission electron microscope(TEM) was used to observe cellular lipofuscin in neuron.Automatic image analysis system for biomedical application was used to determine in quantity SOD and lipofuscin. RESULTS:Learning and memory of AD mice were significantly decreased(P< 0.01).Tiantai I improved significantly learning and memory disturbance of mice in the model of spontaneous AD(P< 0.05-0.01),markedly enhanced SOD activity in central cerebral cortex and hippocampus(P< 0.05-0.01) and reduced cellular lipofuscin content of neuron(P< 0.05-0.01).In addition,it provided certain of quantitative reaction. CONCLUSION:For the mice with spontaneous senile dementia,learning and memory as well as SOD activity of neuron in relevant cerebral areas are decreased remarkably and lipofuscin is increased significantly.Tiantai I acts significantly in pharmacodynamics on anti injury of oxygenic free radical,increases significantly SOD activity, decreases lipofuscin content and improves the ultrastructure of neuron in cortex and hippocampus in AD mice.
出处
《中国临床康复》
CSCD
北大核心
2005年第13期178-181,共4页
Chinese Journal of Clinical Rehabilitation
基金
广东省优秀中青年专家科研目标责任项目(98036)~~
