摘要
目的 探讨5种不同厂家酶免疫试剂的灵敏度和联合应用的必要性。方法 将2 0份抗 HCV阳性标本按1∶10 0～1∶15 0 0不同倍数进行稀释,用5种进口与国产酶免疫试剂检测不同稀释倍数抗 HCV阳性标本。结果 2 0份阳性标本经5种不同厂家试剂检测,结果均为阳性,无一漏检。随着2 0份血样稀释倍数增加,阳性标本检出数和检出阳性标本的试剂数逐渐减少,当稀释倍数达到1∶110 0～1∶130 0时,国产与进口试剂在灵敏度上的差异有显著性(P <0 .0 1)。结论 献血者初复检宜分别选用灵敏度不同的国产和进口试剂联合检测,以确保检测结果的可靠性。
Objective To evaluate the sensitivity of five different anti-HCV enzyme immunoassay kits on the screening of positive blood samples.Methods The positive samples, diluted with different power from 1∶100～1∶1 500, were detected with different import and domestic EIA kits.Results Before diluting twenty samples can be detected with all five different kits. With the increasing of diluting power, the numbers of positive samples and detected EIA kits were decreased.There was significant difference on detection of antibodies between import and domestic kits when diluting power was 1∶1 100～1∶1 300. Conclusion To ensure the reliability of screening of blood donors, the selection of anti-HCV EIA kits should be carried out in blood centers according to the different sensitivity of import and domestic kits in primary and second screening.
出处
《临床输血与检验》
CAS
2005年第2期95-96,共2页
Journal of Clinical Transfusion and Laboratory Medicine
