摘要
目的:探讨脑心通对大鼠局灶性脑缺血损伤后脑组织补体C3表达的影响。方法:参照longa等方法制成大鼠局灶性大脑中动脉阻断(MCAO)模型,将大鼠分为假手术组、生理盐水组、大剂量脑心通组、中剂量脑心通组、小剂量脑心通组,分别给予不同剂量药物,于术后6h,24h,48h,72h和7d进行神经功能评分(NDS),处死后取脑组织观察脑含水量的变化,病理切片HE染色观察组织病理学改变,免疫组织化学的方法测定补体C3的动态变化。结果:脑心通治疗组的神经功能评分均降低,病理损伤均减轻,大、中剂量脑心通组治疗后脑水肿明显减轻(P<0.05),补体C3的表达明显减少(P<0.05)。结论:脑心通可能通过减轻脑水肿,降低脑内补体C3的表达对大鼠缺血脑组织损伤产生保护作用。
Objective: To investigate the effect of Naoxintong (NXT) on complement 3 expression of focal cerebral ischemia (FCI) in rats. Methods: The FCI model with thread embolism of middle cerebral artery occlusion (MCAO) in rats was made. Rats were randomly divided into five groups, sham operation group, normal saline group, high dose treatment group, middle dose treatment group, low dose treatment group. At the time of 6h, 24h, 48h, 72h and 7d after MCAO, the neurological deficit score (NDS) was assessed; the changes of brain water content were measured; the changes of histopathology were observed by the HE staining method; adopting immunohistochemistry method to detect the changes of complement 3(C3) expression. Results: The NDS were all increased and the histopathology damnifications were all alleviated in the NXT treatment groups. Brain edema was reduced obviously (P<0.05) and the expression of C3 was decreased obviously (P<0. 05) in the middle group and the high group. Conclusion: The result showed that NXT had significant protective effects by alleviating brain edema and reducing the expression of C3 in the brain in FCI rats.
出处
《脑与神经疾病杂志》
2005年第2期107-110,共4页
Journal of Brain and Nervous Diseases
关键词
脑心通
补体C3
脑缺血
大鼠
Naoxintong complement 3 cerebral ischemia rats
