摘要
目的 检测诺帝对实验性糖尿病大鼠视网膜胶质细胞反应的影响,并探讨其可能的意义。方法 采用链脲佐菌素诱导的大鼠糖尿病模型,将动物随机分为糖尿病组(10只)、诺帝治疗组(10只)和正常对照组(10只) ,其中诺帝治疗组在出现糖尿病表现后按2 7mg·kg-1腹腔注射诺帝溶液(隔日1次) ,糖尿病组只在同期注射等量生理盐水,正常对照组不给予任何药物。注射后1个月、3个月后取视网膜制备切片,采用免疫组织化学方法定量检测视网膜中胶质纤维酸性蛋白(glialfibrillaryacidicprotein ,GFAP)阳性反应及其分布。结果 腹腔注射后1个月,各组大鼠视网膜GFAP阳性反应多局限在内界膜,阳性面积无显著性差异(P >0 .0 5 ) ;在注射后3个月糖尿病组大鼠GFAP阳性反应增强,贯穿于视网膜全层,阳性反应面积与对照组和诺帝处理组相比,差异有统计学意义(P <0. 0 1) ,而诺帝处理组与对照组大鼠视网膜表达的GFAP仍多局限在内界膜,两者相比差异无明显统计学意义(P >0 .0 5 )。结论 诺帝可抑制实验性糖尿病大鼠视网膜GFAP阳性反应,提示诺帝在一定程度上可减轻视网膜增生性胶质细胞反应。
Objective To observe the effects and significance of Nordy on the expression of glial cells reaction in the retinas of diabetic rats.Methods Streptozotocin induced diabetic rats were divided into diabetes(10 rats) and Nordy groups(10 rats).Other 10 normal rats were recruited as control groups.The Nordy groups were injected 0.5% with Nordy(27 mg·kg^(-1)) while the control and diabetes groups were injected with saline into peritoneal cavity once per 2 days.One month or 3 months later,5 rats in every group were killed and the expression of glial fibrillary acidic protein (GFAP) in the retinas were detected by immunohistochemistry.The average positive area were measured and analysed by computer aided video system.Results In 1 month ,the expression of GFAP in the retinas were all limited in the inner limited membrane.The average positive area had no obvious difference among all groups(P>0.05).In 3 months,in diabetes group,the expression of GFAP in the retinas were transfixed the retinas.Compared with the control and Nordy groups,there had significant difference in average positive area (P<0.01); In control and Nordy group,the expression of GFAP in the retinas were almost limited in the inner limited membrane.There had no obvious difference between the two groups(P>0.05).Conclusion Nordy injected intraperitoneally may be inhibit the expression of GFAP in retinas of diabetic rats and indicate that Nordy could suppress the proliferation of glia cells in retinas.Our data shows that Nordy has potential in the treatment of diabetic retinopathy.
出处
《眼科新进展》
CAS
2005年第2期116-119,共4页
Recent Advances in Ophthalmology
基金
国家高技术研究发展计划 (863计划 )基金资助 (编号 :2 0 0 2AA0 0 1 0 1 0 )~~
