摘要
授粉90d的五唇兰种子95%具球形胚,无菌条件下播种于培养基3个月后种子最高萌发率达到 90%。ABA和高浓度的NH4NO3抑制种子的萌发。NAA和BA促进种子的萌发,最适浓度为BA0.2mg· L 1+NAA0.5mg·L 1。400mg·L 1的谷氨酰胺可促进原球茎生长。2000mg·L 1的蛋白胨促进原球茎发 育成苗。
After pollinating for 90 days,Doritis pulcherrima seeds which 95% contain globular embryos were collected and sowed on medium axenically. After 3 months,the seed germination rate reached 90%. ABA and high concentration of NH 4NO 3 inhibited the germination. NAA and BA greatly improved the germination and the combination of BA 0.2 mg·L^(-1) and NAA 0.5 mg·L^(-1) was optimal. Glutamine 400 mg·L^(-1) made protocorms grow well while peptone 2000 mg·L^(-1) promoted the formation of seedlings.
出处
《广西植物》
CAS
CSCD
北大核心
2005年第2期149-151,i004,共4页
Guihaia
基金
国家自然科学基金(30170061)
广东省重大科技专项(2003A2010401)
福建省青年科技人才创新基金项目(2004J052)资助
