摘要
目的: 探讨黄芪与当归对内皮细胞(ECs)增殖周期及血管内皮细胞生长因子(VEGF)表达的影响。方法: 以体外培养人脐静脉内皮细胞(HUVEC)为模型,HUVEC经分离、原代和传代培养与鉴定,选择第4 或第5代细胞进行实验;MTT法检测不同剂量黄芪与当归对细胞增殖的影响;流式细胞仪检测黄芪与当归对HUVEC增殖周期的影响;半定量RT PCR检测黄芪与当归对HUVEC的VEGF mRNA表达的影响。结果:细胞周期检测显示黄芪及当归组S期细胞数显著增多(P<0.01)。黄芪+当归组G0 G1 期细胞数明显减少(P<0.05),S期和G2 M期细胞数明显增多(P<0.05)。RT PCR结果表明,黄芪与当归使HUVEC的VEGF mRNA表达明显增强,黄芪+当归(200μg/ml)组是对照组的1.82倍;当归(200μg/ml)组是对照组的1.88倍;黄芪(200μg/ml)组是对照组的1.51倍。结论:黄芪与当归及其复方具有促进内皮细胞增殖作用,黄芪+当归可明显促进HUVEC的DNA合成及有丝分裂。黄芪与当归可促进HUVEC的VEGF mRNA表达,提示黄芪与当归通过刺激VEGF表达而促血管新生。
Objective: To investigate the effects of astragalus membranaceus and angelica sinensis on the proliferation of endothelial cells, cell cycle and the expression of vascular endothelial growth factor(VEGF) mRNA in human umbilical vein endothelial cells (HUVEC ). Methods: To culture human umbilical vein endothelial cells as target cells in vitro. Factor Ⅷ-related antigen determined was masculine in the cultures, with monoclonal antibody and SABC immunohistochemistry kit. The proliferation of HUVEC in different concentrations of astragalus membranaceus, angelica sinensis, that include 100, 200, 400 μg/ml,were observed with MTT method. The changes of cell cycle of the cultures after administration of astragalus membranaceus, angelica sinensis in 200 μg/ml were observed using flow cytometer. The decoction of them on VEGF mRNA expression of HUVEC were observed by using RT-PCR method. Results: The contribution of astragalus membranaceus in 200 μg/ml concentration and the decoction in 200, 400 μg/ml concentration was most evident. The decoction of astragalus membranaceus and angelica sinensis enhanced the synthesis of DNA and mitosis in cultured endothelial cells. The cultures treated with astragalus membranaceus, angelica sinensis, and the decoction in 200 μg/ml showed increased VEGF gene expression respectively as compared with negative control. Conclution: Astragalus membranaceus, angelica sinensis, and the decoction of them promoted proliferation of cultured HUVEC in vitro, increased the mRNA and expression of VEGF in the cultured HUVEC.
出处
《新疆医科大学学报》
CAS
2005年第3期224-227,共4页
Journal of Xinjiang Medical University
关键词
内皮细胞
内皮细胞生长因子
细胞增殖
黄芪
当归
vascular endothelial growth factor
astragalus memb- ranaceus
angelica sinensis
endothelial cells
proliferation
