摘要
目的:探讨创伤应激状态下小鼠免疫功能变化及黄芪多糖(APS)对应激状态下机体免疫功能影响。方法:建立小鼠截肢应激模型,将50只Balb/c小鼠随机分为5组: 正常对照组(A);应激对照组(B);应激+ 黄芪多糖高(C)、中(D)、低(E)剂量组,每组10只。创伤后72小时称小鼠胸腺、脾脏湿重,用原位杂交检测胸腺、脾脏淋巴细胞中NF-κB mRNA、IL-10mRNA表达,计算机图像分析系统定量。结果:与正常对照组(A)比较,创伤后72小时应激对照组(B)小鼠胸腺、脾脏重量指数明显下降(P<0.01=;胸腺、脾脏淋巴细胞NF-κBmRNA、IL-10mRNA表达水平均明显升高(P<0.01);与创伤应激对照组(B)比较,应激+ 黄芪多糖高(C)、中(D)、低(E)剂量组小鼠胸腺、脾脏指数有不同程度的升高,胸腺、脾脏淋巴细胞中NF-κB mRNA、IL-10mRNA的表达受抑(P<0.01)。与正常对照组(A)比较,应激+ APS高剂量组(C)小鼠胸腺、脾脏指数;胸腺、脾脏组织中NF-κB mRNA、IL-10mRNA的表达无显著性差异(P>0.01>0.05)。结论:创伤后小鼠免疫功能受抑;黄芪多糖体内应用可有效恢复其免疫功能。
Objectives: To evaluate the effects of traumatic stress on mice cellular immunity, and the influences of Astragalus Polysaccharide (APS) on the mice cellular immunity. Methods: A stress model of mouse amputed was established, and 50 Balb/C mice were divided randomly into 5 groups (10 mice each group): group A (normal control group), group B (traumatic stress group), groupC (traumatic stress+high dose APS group),group D (traumatic stress+middle dose APS group) and group E(traumatic stress+low dose APS group). The thymus and spleen of these mice stressed were weighted after 72 hours, and the expression of NF-闎 mRNA , IL-10 mRNA in lymphocytes from both tissues were detected by situ hybridization. All results were quantitated by HPIAS-1000 system. Results: Compared with group A, the weight data were decreased significantly in group B (P<0.01), and the expression of NF-闎 mRNA and IL-10 mRNA in lymphocytes was increased evidently(P<0.01). Compared with group B, the weight data were elevated with different degree in groups C,D and E, and the expression of NF-闎 mRNA and IL-10 mRNA in lymphocytes were reduced (P<0.01). In group A and group C, the weight data and the expression of NF-B mRNA and IL-10 mRNA in lymphocytes of thymus and spleen had no significant difference (P>0.01>0.05). Conclusion: The mice cellular immunity function was inhibited after their trauma, but APS may restore the function effectively.
出处
《中国体视学与图像分析》
2004年第1期21-24,共4页
Chinese Journal of Stereology and Image Analysis
