摘要
目的 研究大豆胚轴提取物对糖尿病大鼠血及肝脏过氧化脂质 (LPO)水平、丙二醛 (MDA)含量及超氧化物歧化酶 (SOD)活性的影响。方法 给糖尿病大鼠喂饲添加 2 0 g/kg大豆胚轴提取物的饲料 2 0周后 ,分离血清 ,制备肝匀浆。采用八木变法测定过氧化脂质含量 ,采用硫代巴比妥酸法测定MDA含量 ,采用黄嘌呤氧化酶法测定SOD活力。结果 食用大豆胚轴提取物的糖尿病大鼠 ,其血清LPO水平和MDA含量显著降低 ,肝脏及血清SOD活性明显升高。结论 大豆胚轴提取物可降低糖尿病大鼠肝脏及血清中脂质过氧化程度和提高SOD活性 。
Objective To investigate the effect of soybean hypocotyl extract on lipid peroxide (LPO),malondialdehyde(MDA) and superoxide dismetase(SOD) of blood and liver in diabetic rats.Methods Diabetic rats were given diet containing 20?g/kg of soybean hypocotyl extract for 20 weeks,and then blood LPO was measured by colorimetric method,MDA was measured by thiobarbituric method,and SOD activity was investigated by xanthine oxidase method.Results After 20 weeks of experiment,serum LPO and MDA contents were significantly decreased,and SOD activities in serum and liver were increased noticeably.Conclusion Soybean hypocotyl extract had the function of decreasing blood LPO and MDA contents,and increasing serum and hepatic SOD activities,thus it may be useful in prevention and treatment of diabetes mellitus and diabetes-associated diseases.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第11期1293-1294,共2页
Chinese Journal of Public Health
基金
国家自然科学基金资助项目 (30 360 1 1 3)
关键词
大豆
过氧化脂质
丙二醛
超氧化物歧化酶
糖尿病
soybean
lipid peroxide
malondialdehyde
superoxide dismetase
diabetes mellitus
