摘要
目的:探讨哮喘大鼠肺组织中核因子鄄κB(NF鄄κB)与基质金属蛋白酶鄄9(MMP鄄9)及金属蛋白酶组织抑制因子鄄1(TIMP鄄1)的关系以及地塞米松对NF鄄κB、MMP鄄9和TIMP鄄1的作用。方法:建立大鼠哮喘模型,采用免疫组化法观察肺中NF鄄κB活化及MMP鄄9、TIMP鄄1的蛋白表达,用逆转录-聚合酶联反应技术(RT鄄PCR)测定肺中MMP鄄9、TIMP鄄1mRNA的表达。结果:哮喘组NF鄄κB的活化及MMP鄄9、TIMP鄄1蛋白在气道黏膜上皮的表达明显高于对照组(P<0.01)和地塞米松组(P<0.05),哮喘组MMP鄄9、TIMP鄄1在肺组织的mRNA表达也高于对照组(P<0.01)和地塞米松组(P<0.01)。MMP鄄9的表达与NF鄄κB的活化呈显著正相关(rs=0.8071,P<0.01)。TIMP鄄1的表达与NF鄄κB的活化无明显相关(rs=0.4716,P>0.05)。结论:地塞米松可能抑制气道壁NF鄄κB活化和减少MMP鄄9的表达、调节MMP鄄9/TIMP鄄1之间的失衡,对MMP鄄9的抑制作用可能部分通过抑制NF鄄κB活化而实现的。
Objective:To observe the relationship among the expressions of nuclear factor-κB(NF-κB),metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) and the effects of dexamethasone in the lung of asthmatic rats. Methods: Asthmatic model was established by using rats. The NF-κB activity and the protein expressions of MMP-9 and TIMP-1 were examined by immunohistochemical analysis. The expressions of MMP-9 and TIMP-1 mRNA in the lung were assessed by reverse transcription-polymerase chain reaction(RT-PCR). Results:The NF-κB activity and the protein expressions of MMP-9 and TIMP-1 in bronchial epithelum in the asthmatic group were higher than those in the control group(P < 0.01) and the dexamethasone group(P < 0.05). The mRNA expressions of MMP-9 and TIMP-1 were significantly increased than those in the control group(P < 0.01) and in the dexamethasone group(P < 0.01). There was a significantly positive correlation between the expression of MMP-9 and the activity of NF-κB,but not found between the expression of TIMP-1 and the activity of NF-κB. Conclusion:Dexamethasone may downregulate the activity of NF-κB and the expression of MMP-9 and may regulate the balance of MMP-9/TIMP-1. The effect of Dexamethasone on NF-κB may partly influence the expression of MMP-9.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期255-258,261,共5页
Journal of Nanjing Medical University(Natural Sciences)
