摘要
以 No.2 4菌株为出发菌株 ,采用紫外线、化学试剂、微波及超声波诱变法对其进行诱变。其中经微波诱变 5 0 s后 ,筛选得到一株高产菌株 ,命名为 Ms-2 4菌株。在培养基中连续培养 1 0代 ,其遗传性状非常稳定。Ms-2 4菌株发酵试验证明 ,其发酵产量比出发菌株提高 2 3.2 5 % ;抗菌活性试验证明 ,其发酵液的抗菌活性比出发菌株提高了 31 .75 %。
As a original strain, Streptomyces spp. Xinong No.24 was mutated by UV, chemicals, microwave irradiation and ultrasonic. A high producing antibiotic strain named Ms 24 was obtained through the microwave irradiating 50 s. Successively cultured ten times in the medium, heredity character of Ms-24 was stable.The result of fermentation test showed that the fermentation yield increased by 23.25%,The result of antifungal activity test indicated that the activity increased by 31.75%.
出处
《西北农业学报》
CAS
CSCD
北大核心
2005年第1期98-101,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家 8 6 3高技术计划项目 ( 2 0 0 2 A A2 45 1 2 1 )
关键词
No.24菌株
诱变选育
农用抗生素
发酵
No.24 strain
Breeding
Ultraviolet (UV)
Chemicals
Microwave
Ultrasonic
Mutation
