摘要
本文建立了贝类产品中诺沃克病毒检测的普通RT -PCR方法。用 8对引物对诺沃克病毒进行检测研究 ,发现GII-SKF/GII-SKR引物检测GII型病毒特异性强且灵敏度高。用引物GI-SKF/GI-SKR和GII-SKF/GII-SKR分别对系列稀释度的质粒进行检测 ,检测灵敏度均能达到 10 2拷贝。将诺沃克病毒添加于牡蛎肠胃组织中并提取RNA ,并用引物GI-SKF/GI-SKR和GII-SKF/GII-SKR对其进行扩增 ,检出GII型诺沃克病毒。
A reverse transcription PCR system was established for the detection of Noroviruses in shellfish. Four GII Norovirus strains were detected by eight primer sets, and it was found that GII-SKF/GII-SKR was highly sensitive and specific. The 10-fold serial dilutions of GI and GII cloning plasmids including the target sequences were amplified by the GI-SKF/GI-SKR and GII-SKF/GII-SKR primer sets, respectively, and it was found that the detection limits were as low as 102 copies of target fragment. The seeded oysters were assayed by the above RT-PCR method, and the GII Norovirus was detected.
出处
《检验检疫科学》
2005年第1期39-42,共4页
Inspection and Quarantine Science
基金
国家标准专项上海地方实施项目 (课题编号 :0 2dz0 5 0 3 9)
国家质检总局科研项目 (课题标号 :2 0 0 4IK0 72 )
