摘要
利用 RT- PCR扩增了口蹄疫病毒 (FMDV )的 RNA复制酶基因 ,并将其克隆到原核表达载体 p ET- 32 a(+)中。重组质粒在大肠杆菌中表达后的目的蛋白为可溶性形式 ,纯化产物用感染 A型 FMDV的豚鼠康复血清进行 Western-blotting检测 ,结果表明 。
The RNA replicase gene of foot-and-mouth virus was amplified and cloned into prokaryotic expression vector pET-32a(+).The recombinant plasmid was transformed into E.coli and the target protein was expressed in soluble form.The purified recombinant protein was substantiated to be expressed correctly by Western-blotting.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2005年第2期119-121,共3页
Chinese Journal of Veterinary Science
