摘要
目的 :建立较理想的大鼠肾小管上皮细胞原代培养、传代及鉴定方法。方法 :采用肾小管节段贴块法及 0 .2 %胰蛋白酶消化 2 0 min两种方法进行原代培养 ,以 0 .2 5 %胰蛋白酶 (A组 )、0 .12 5 %胰蛋白酶 - 0 .0 2 % EDTA(B组 )消化传代 ,利用免疫细胞化学方法鉴定细胞种类。结果 :两种方法均能成功培养肾小管上皮细胞 ,但前者较好 ,小管节段贴壁早。B组成功传代 (4代 )并鉴定为肾小管上皮细胞 ,A组传代失败。结论 :肾小管节段贴块、0 .12 5 %胰蛋白酶 - 0 .0 2 % EDTA消化是大鼠肾小管上皮细胞原代培养及传代的有效方法。
Objective:To establish a practical method for primary and passage cultures in rat renal cells. Methods: Both segmental sticking of renal tubule from SD rats and digestion with 0.2% trypsin for 20 minutes were used in primary culture. Digestion with 0.25% trypsin (Group A) or with 0.125% trypsin plus 0.02% EDTA (Group B) was applied in passage culture. Cell types were identified by immunocytochemistry. Results: Two methods for primary culture were successful, but the segmental sticking was better. The passage culture was only seen in Group A, and 4 generations were achieved. Conclusion: The segmental sticking of renal tubule and digestion with 0.125% trypsin plus 0.02% EDTA are eligible methods for primary and passage cultures in rat renal cells, respectively.
出处
《广东医学院学报》
2005年第1期10-13,共4页
Journal of Guangdong Medical College
基金
广东医学院博士启动基金 (2 0 0 2年 )
关键词
肾小管上皮细胞
细胞培养
免疫细胞化学
renal tubular epithelial cell
cell culture
immunocytochemistry
