摘要
研究了菌龄、酶系统、脱壁促进剂、渗透压稳定剂对酿酒酵母ＲａｓｓｅⅦ（ＳａｃｃｈａｒｏｎｍｙｃｅｓｃｅｒｅｖｉｓｉａｅＲａｃｅⅫ）原生质体形成与再生的影响。结果表明，对数生长早期的菌体最适于制备原生质体；酶浓度是影响原生质体产量的主要因子，其次为时间，再次是温度；而就再生率而言，温度则是决定因子，酶浓度为第二，时间则几乎不起作用；脱壁促进剂能够加速细胞壁的溶解，尤以巯基乙醇效果较好，然而也有降低再生率之弊，综合考虑原生质体形成与再生的情况，本研究中以选用２％的酶浓度３０℃下酶解１－１．５ｈ于１７％蔗糖高渗培养基上再生为获得ＲａｓｓｅⅫ原生质体形成率和再生率的最佳条件。
Effect of the culture age, the enzyme system, the pretreatment and the osmotic stablizers on the formation and regeneration of protoplasts from S. cerevisiae were studied. The optimal condition of isolating protoplasts of Race Ⅶ was that protoplasts from the culture of early exponential phase were released by snail enzyme as a lytic enzyme at 30℃ for go minutes and the protoplasts were regenerated on the medium co4taining 17% sucrose as asmotic stablizer.
出处
《微生物学杂志》
CAS
CSCD
1994年第2期12-16,11,共6页
Journal of Microbiology
