摘要
植物开花机理是生物学中的一个基本问题,多年来人们进行过许多的研究,积累了大量的事实,然而对开花的机理仍然还不甚清楚。因而在利用原有实验系统的同时,有必要寻找更多简单,又便于分析的实验系统。Jullien等报告离体培养的大豆子叶节能直接产生花芽。我们在建立离体培养黄瓜子叶直接单独形成雄花或雌花的实验系统的过程中,发现黄瓜幼苗去除顶芽后在子叶节处也能直接形成花芽。
The effects of KNO3 and paclobutra-zol (PP333) on direct floral bud formation at the cotyledonary node region of the decapitated cucumber seedlings cultured with nutrient solution of low strength have been studied. Direct formation of the floral buds was found to be closely correlated with KNO3 level. When nutrient solution was supplemented with 0.01 mmol/L KNO3, the higher percentage of direct floral bud formation i. e. 38.33% was got. Effect of PP333 on direct floral budformation was more significant, and the higher percentage i. e. 73.33% was got when nutrient solution was supplemented with 0.75mg/L PP 333. The foliar sprays of 500 ppm PP 333 stimulated floral bud formation not as effectively as supplement of PP 333 to nutrient solution, the percentage of direct floral bud formation was 45% only. The results suggest that this experimental system would be valuable in studying the mechanism of floral evocation and development.
出处
《实验生物学报》
CSCD
1994年第3期359-365,共7页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
浙江省自然科学基金
关键词
黄瓜
去顶
硝酸钾
多效唑
花芽
Cucumis sativus L. Decapitation. Potassium nitrate. Paclobutrazol (PP 333). Floral bud formation.
