摘要
用黄瓜花叶病毒接种矮牵牛，取发病植株的叶片、花芽和茎尖进行培养，用间接ＥＬＩＳＡ和离体蚕豆叶片进行检测，结果表明：用叶片、花芽以及大于０．３～０．５ｍｍ的茎尖培养，不能获得无毒苗，从病株上剥取０．１５～０．２５ｍｍ的茎尖培养可获得６．６％的无毒苗；连续高温处理病株８ｄ结合茎尖（０．３～０．５ｍｍ）培养，可获１０％的无毒苗；分别变温处理（３８℃８ｈ＋２２℃１６ｈ）１６ｄ和３０ｄ结合茎尖（０．３～０．ｓｍｍ）培养，均可获得１１．１％的无毒苗。
The leaves, flower buds and meristem-tips from the infected petunia plants inoculated with CMV were cultured and the virus in calli and plantlets was checked by indirect ELISA and by inoculation on detached broad-beau leaves. The results showed as follows: (1)virus-free plantlets were not obtained as the leaves, flower-buds and meristem-tips (more than 0. 3mm) were cultured; (2) 6. 6% plantlets were apparently free from CMV as 0. 15～0. 25mm meristem-tips were cultured; (3) 10% virus-free plantlets were produced from the cultures of meristem-tips(0. 3-0. 5mm) from the infected plants that had been continuously treated at 40℃ for & days; (4)the same percent (11. 1%) of virus-free plantlets were obtained as the meristem-tips (0. 3～0. 5mm)were cultured from the infected plants that had been treated with alternating temperature in diurnal circles 38℃ 8h+22℃ 16 h for 16 or 30 days, respectively.
出处
《上海农业学报》
CSCD
1994年第2期71-74,共4页
Acta Agriculturae Shanghai
