摘要
本文以催化动力学分析法为监测手段，利用Ｍｎ￣（２＋）离子对ＫＩＯ＿４－ＲｈＢ反应体系的催化作用，在使用ｐｈｅｎ为活化剂的条件下，顺利实现对兔全血中微量锰的荧光测定，绘制药时曲线，效果良好。
A Catalytic k inetic fluorometric methed for the determination of microamounts of manganase has been developed based on the catalytic effect of Mn on the oxidation of rhedamine B by KIO_4 by using o-phenanthroline as the activator.The relative standard deviation is 3.62% at the concentration level of 4×10￣(-9)g/mL with a detection limit of 4.3×10￣(-10)g/mL,The average recovery is 99.76%.This method has been used for the establishment of the drug- time curve in rabbit blood.The mechanism of the catalytic reaction has also been discussed.
出处
《分析试验室》
CAS
CSCD
北大核心
1994年第1期20-23,共4页
Chinese Journal of Analysis Laboratory
基金
国家中医药管理局科研基金
关键词
催化动力学
锰
药时曲线
兔
荧光
Catalytic kinetic fluorometry
manganese determinaion
drug-time curve
rabbit blood analysis
