摘要
本文建立了人血浆中汉防己甲素的高效液相色谱测定法。采用C18柱 ,以含 0 0 3%三乙胺的甲醇∶水 (80∶2 0 )为流动相 ,检测波长 2 82nm ,安定为内标 ,样品用乙醚提取。汉防己甲素线性范围 0 2 89～ 4.6 18μg·ml-1,平均加样回收率 95 8% ,日内及日间变异少于 5 %。方法简便、快速、可靠 ,可用于汉防己甲素的生物样本分析。
The paper reports the determination of tetrandrine by high performance liquid chromatography(HPLC). A C 18 column was used and the mobile phase was methanol water(80∶20) containing 0.03% triethylamine, detective wave was at 282?nm, the internal standard was diazepam, the sample was extracted with ether. The linear range was from 0.289?μg·ml -1 to 4.618?μg·ml -1 , the average recovery rate was 95.8%. The coefficients of variation of within day and day to day were less than 5%. This method is simple, rapid and accurate. It can be used in biomedical sample analysis of tetrandrine. [
出处
《湖南医科大学学报》
CSCD
2000年第4期409-410,共2页
Bulletin of Hunan Medical University
基金
湖南省科委资助!项目 ( 0 2 961 0 1 2 )
关键词
汉防己甲素
色谱法
高效液相
方法
tetrandrine
high performance liquid chromatography
method
