摘要
目的建立高效液相色谱法测定不同产地大蒜中蒜氨酸的含量。方法采用色谱柱ZirchromODS柱,流动相甲醇水(3∶7)为,检测波长214nm。结果蒜氨酸进样量在0.0103～0.5151mg·mL-1范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率98.7%。日内精密度RSD为1.1%,日间精密度RSD为1.9%。结论实验表明该法简便、快速、适用性好。
AIM: To establish a RP HPLC method to determine the content of alliin in Allium sativum Linn. METHODS: Chromatographic separation was performed on ODS column with a mobile phase composed of methanol water (3∶7 by volume). The eluate was monitored at 214nm wavelength. RESULTS: The average recovery of sample for alliin was 98.7%, The RSD of within day was 1.1%. The RSD of between day was 1.9%. CONCLUSION: The method is simple, rapid with good suitability.
出处
《中成药》
CAS
CSCD
北大核心
2004年第12期1025-1027,共3页
Chinese Traditional Patent Medicine
关键词
蒜氨酸
大蒜
HPLC
alliin
Allium sativum Linn
HPLC
作者简介
常军民(1965～),男,陕西大荔人,副教授,博士,研究方向:天然药物的研究与开发,电话:0991-4362471,E-mail:CJMCJN2471@yahoo.com.cn.
