摘要
本文用自制的寡核苷酸探针(CAC)_5/(GTG)_5,对与法医学应用有关的问题进行了研究。从室温下放置三年的血痕得到不完全的DNA指纹图;从同一个体不同组织的DNA得到完全一致的结果;从混合斑中分离出精子DNA进行DNA指纹检验,并与同一供体的血液DNA指纹图进行比较;还用仅有0.25μg的DNA获得了可分辨的DNA指纹图。此外,用限制性内切酶HaeⅢ代替HinfⅠ酶切人的基因组DNA,也获得了高度多态性的杂交图谱。结果表明,(CAC)_5/(GTG)_5是一种具有实用价值的DNA指纹探针。
The synthetic oligonucleotide probe (CAC)5/(GTG)5 for DNA, fingerprinting was studied in this paper. The imcomplete pattern of DNA fingerprints was obtained from bloodstains up to three years old, which were kept at room temperature. The identical results were achieved from various human organ tissues of the same individual. DNA fingerprints from vaginal swabs was the same as that obtained from the male donor's blood. The discernible DNA fingerprints were obtained with as little as 250ng of genomic DNA. Besides, using restriction enzyme Hae Ⅲ instead of Hint I to digest human DNA, the highly polymorphic hybridization pattern was also obtained. A practical case was tested by Hint Ⅰ and Hae Ⅲ digestion, respectively. The results demonstrated that the (CAC)5/(GTG), was a suitable probe for DNA fingerprinting in forensic practice.
关键词
寡核苷酸
探针
法医学
DNA
肤纹
Oligonucleotide probe, DNA fingerprinting, forensic biology, individual specificity
